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STD Testing in National City, California

5 STD and HIV testing locations in National City, including 3 that list free testing.

Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.

Family Health Centers of San Diego – National City Family Health Center

1000 Euclid Ave, National City, CA 91950 · (619) 515-2399
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:30am – 5:30pm; Tue 8:30am – 5:30pm; Wed 8:30am – 5:30pm; Thu 8:30am – 5:30pm; Fri 8:30am – 5:30pm

La Maestra Community Health Centers – National City Clinic

217 Highland Ave, National City, CA 91950 · (619) 280-4213
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

OPSAM Health – National City Medical

2743 Highland Ave, National City, CA 91950 · (844) 200-2426
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm; Sat 8:00am – 5:00pm

San Ysidro Health – National City Health Center

601 E 14th St, Ste 201, National City, CA 91950 · (619) 662-4100
Sliding scaleChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis C
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

San Ysidro Health – Paradise Hills

2400 E 8th St, National City, CA 91950 · (619) 662-4100
Sliding scaleChlamydiaGonorrheaSyphilisHIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

Community health centers in National City

These federally funded health centers (HRSA) offer primary care, usually on a sliding fee scale. Their STD testing services are not confirmed in our data. Call to confirm STD testing.

OPSAM HEALTH: Mobile Clinic 2

1428 Highland Ave, National City, CA 91950 · (844) 200-2426
Operation Samahan, Inc. · Community health center (HRSA). Call to confirm STD testing.

San Ysidro Health National City Family Clinic

1136 D Ave, National City, CA 91950 · (619) 662-4100
Centro De Salud De La Comunidad De San Ysidro, Inc. · Community health center (HRSA). Call to confirm STD testing.

San Ysidro Health South Bay

330 E 8th St # 340, National City, CA 91950 · (619) 662-4100
Centro De Salud De La Comunidad De San Ysidro, Inc. · Community health center (HRSA). Call to confirm STD testing.

Frequently asked questions

Where can I get STD testing in National City, CA?

This page lists 5 STD/HIV testing locations in National City, CA from the CDC NPIN directory, plus 3 community health centers that may offer testing. Call ahead to confirm services and hours.

Is there free STD testing in National City, CA?

3 of the 5 listed locations say they offer free STI and/or HIV testing. Eligibility can depend on age, income or residency. Many health departments and community health centers also use sliding-scale fees.

Do I need an appointment?

Appointment rules vary by location. Call before you go.

Can I get a rapid HIV test?

2 locations here list rapid HIV testing, which usually gives results during the same visit.

Which tests should I ask for?

Common tests include chlamydia, gonorrhea, syphilis, HIV and sometimes herpes or hepatitis. Which ones you need depends on your situation, so a clinician can help you decide. CDC recommends everyone ages 13 to 64 get tested for HIV at least once.

Is STD testing confidential?

Health care providers must keep your health information private. Some public health clinics offer anonymous HIV testing. Ask the clinic about their privacy policy.

Data: CDC NPIN and HRSA. Page built 2026-11-03.