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Florida Department of Health in Hernando County – Brooksville Office
STD/STI testing location in Brooksville, Florida.
Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.
Address: 300 S Main St, Brooksville, FL 34601
Get directions
Phone: (352) 540-6800
Website: https://hernando.floridahealth.gov/
Tests listed
- Chlamydia Test
- Conventional HIV Test
- Gonorrhea Test
- Hepatitis A Test
- Hepatitis B Test
- Hepatitis C Test
- Herpes Test
- Rapid HIV Test
- Syphilis Test
- TB Test
Cost and payment
Medicaid Accepted, Sliding Scale, PrEP for uninsured, No Fee, Fee, Insurance Accepted, Free STI Test, Free HIV Test, Free Hepatitis C Test, Free Hepatitis B Test.
This location lists free STI and/or HIV testing. Eligibility rules may apply.
Hours
| Monday | 8:00am – 5:00pm |
| Tuesday | 8:00am – 5:00pm |
| Wednesday | 8:00am – 5:00pm |
Appointment required: Yes
Other services
STI treatment, PrEP (HIV prevention medicine).
Languages: English
Type: Public Health Department/Social Services Department
Source: CDC NPIN listing. Last updated by source: 2026-04-24. Page built 2026-11-03.