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MainStreet Family Care – Alachua Location
STD/STI testing location in Alachua, Florida.
Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.
Address: 16314 NW US Hwy 441, Alachua, FL 32615
Get directions
Phone: (386) 518-2418
Website: https://www.mainstreetfamilycare.com/locations/alachua/
Tests listed
- Chlamydia Test
- Conventional HIV Test
- Gonorrhea Test
- Hepatitis B Test
- Hepatitis C Test
- Herpes Test
- Syphilis Test
- TB Test
Cost and payment
Medicaid Accepted, Medicare Accepted, Fee, Insurance Accepted.
Hours
| Sunday | 8:00am – 4:00am |
| Monday | 8:00am – 8:00pm |
| Tuesday | 8:00am – 8:00pm |
| Wednesday | 8:00am – 8:00pm |
| Thursday | 8:00am – 8:00pm |
| Friday | 8:00am – 8:00pm |
| Saturday | 8:00am – 4:00pm |
Appointment required: No (walk-ins may be accepted)
Other services
STI treatment, PrEP (HIV prevention medicine).
Languages: English
Type: Clinic
Source: CDC NPIN listing. Last updated by source: 2025-09-26. Page built 2026-11-03.
Other testing sites nearby
Florida Department of Health in Alachua County – Alachua Clinic
15530 NW US Hwy 441, Ste 10010, Alachua, FL 32615 · (386) 462-2542
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm