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MainStreet Family Care – Callahan Location

STD/STI testing location in Callahan, Florida.

Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.

Address: 542435 US Hwy 1, Callahan, FL 32011
Get directions

Phone: (904) 507-4623

Website: https://www.mainstreetfamilycare.com/locations/callahan/

Tests listed

Cost and payment

Medicaid Accepted, Medicare Accepted, Fee, Insurance Accepted.

Hours

Sunday8:00am – 4:00pm
Monday8:00am – 8:00pm
Tuesday8:00am – 8:00pm
Wednesday8:00am – 8:00pm
Thursday8:00am – 8:00pm
Friday8:00am – 8:00pm
Saturday8:00am – 4:00pm

Appointment required: No (walk-ins may be accepted)

Other services

STI treatment, PrEP (HIV prevention medicine).

Languages: English

Type: Clinic

Source: CDC NPIN listing. Last updated by source: 2025-10-17. Page built 2026-11-03.

Other testing sites nearby

Florida Department of Health in Nassau County – Callahan Clinic

45377 Mickler St, Callahan, FL 32011 · (904) 320-6010
Sliding scaleChlamydiaGonorrheaSyphilisHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Thu 8:00am – 5:00pm