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STD Testing in Jacksonville, Florida

15 STD and HIV testing locations in Jacksonville, including 7 that list free testing.

Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.

APEL Health Services Center Incorporated

728 Blanche St, Ste 213, Jacksonville, FL 32204 · (904) 265-1910
Free testing listedSyphilisHIVRapid HIVHepatitis C
Mon 10:00am – 5:00pm; Tue 10:00pm – 5:00pm; Wed 10:00am – 7:00pm; Thu 10:00am – 5:00pm; Fri 10:00am – 7:00pm

CAN Community Health – Jacksonville Clinic

4615 Philips Hwy, Jacksonville, FL 32207 · (904) 508-0710
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis C
Mon 8:30am – 5:30pm; Tue 8:30am – 5:30pm; Wed 8:30am – 5:30pm; Thu 8:30am – 5:30pm; Fri 8:30am – 12:30pm

First Coast Community Development

525 N Newnan St, Jacksonville, FL 32202 · (904) 379-8383
Free testing listedHIVRapid HIVHepatitis C
Mon 8:30am – 4:00pm; Tue 8:30am – 4:00pm; Wed 8:30am – 4:00pm; Thu 8:30am – 4:00pm; Fri 8:30am – 4:00pm; Sat 12:30pm – 2:00pm

HOPE Across the Globe – Jacksonville

6817 Southpoint Pkwy, Ste 603, Jacksonville, FL 32216 · (904) 374-4500
Free testing listedChlamydiaGonorrheaSyphilisHIVHepatitis C
Mon 9:00am – 3:00pm; Tue 9:00am – 3:00pm; Wed 9:00am – 3:00pm; Thu 9:00am – 3:00pm; Fri 9:00am – 12:00pm

JASMYN Incorporated

929 Peninsular Pl, Jacksonville, FL 32204 · (904) 389-3857
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIV
Mon 12:00pm – 6:00pm; Tue 12:00pm – 6:00pm; Wed 12:00pm – 6:00pm; Thu 12:00pm – 6:00pm; Fri 12:00pm – 6:00pm

Maxcare Clinic – Normandy Blvd Clinic

5547 Normandy Blvd, Jacksonville, FL 32205 · (904) 592-9290
Free testing listedChlamydiaGonorrheaSyphilisHerpesHIVRapid HIV
Mon 10:00am – 2:00pm

Midway Specialty Care Center – Jacksonville

3938 Sunbeam Rd, Ste 3, Jacksonville, FL 32257 · (904) 747-2025
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 10:00am – 6:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm

AIDS Healthcare Foundation – Jacksonville Healthcare Center

2 Shircliff Way, Ste 900, Jacksonville, FL 32204 · (904) 381-9651
ChlamydiaGonorrheaSyphilisHIVRapid HIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

Florida Department of Health in Duval County – Comprehensive Care Center at Central Health Plaza

515 W 6th St, Jacksonville, FL 32206 · (904) 253-1040
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIV
Mon 8:00am – 5:00pm; Tue 8:00am – 7:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 7:00pm; Fri 8:00am – 5:00pm

Florida Department of Health in Duval County – South Jacksonville Health Services

3225 University Blvd S, Ste 101, Jacksonville, FL 32216 · (904) 253-2555
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

Mayo Clinic – HIV Clinic

4500 San Pablo Rd, Jacksonville, FL 32224 · (904) 953-0853
ChlamydiaGonorrheaSyphilisHerpesHIV
Hours not listed

Northeast Florida Infectious Disease Consultants

6817 Southpoint Parkway, Ste 802, Jacksonville, FL 32216 · (904) 646-1987
ChlamydiaGonorrheaSyphilisHIVHepatitis CHepatitis B
Mon 8:00am – 3:30pm; Tue 8:00am – 3:30pm; Wed 8:00am – 3:30pm; Thu 8:00am – 3:30pm; Fri 9:00am – 12:00pm

Planned Parenthood of Florida – Jacksonville Health Center

5978 Powers Ave, Jacksonville, FL 32217 · (904) 399-2800
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 4:30pm; Tue 9:00am – 5:00pm; Wed 11:00am – 7:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 4:30pm; Sat 9:00am – 4:00pm

Reza Health

949 Lane Ave S, Jacksonville, FL 32205 · (888) 831-2949
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Hours not listed

UF Health – Womens Specialists - Jacksonville

655 W 8th St, 3rd Fl, Jacksonville, FL 32209 · (904) 383-1037
Sliding scaleChlamydiaGonorrheaSyphilisHIV
Mon 8:00am – 4:30pm; Tue 8:00am – 4:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm

Community health centers in Jacksonville

These federally funded health centers (HRSA) offer primary care, usually on a sliding fee scale. Their STD testing services are not confirmed in our data. Call to confirm STD testing.

AGAPE Community Health Center/West Jacksonville

120 King St, Jacksonville, FL 32204 · (904) 253-1030
Agape Community Health Center, Inc. · Community health center (HRSA). Call to confirm STD testing.

Agape Community Health Center, Inc. - Johnson YMCA

5700 Cleveland Rd, Jacksonville, FL 32209 · (904) 760-4904
Agape Community Health Center, Inc. · Community health center (HRSA). Call to confirm STD testing.

Agape Community Health Center/Blanding

5460 Blanding Blvd, Jacksonville, FL 32244 · (904) 760-4940
Agape Community Health Center, Inc. · Community health center (HRSA). Call to confirm STD testing.

Agape Pharmacy

1680 Dunn Ave STE 38, Jacksonville, FL 32218 · (904) 760-4904
Agape Community Health Center, Inc. · Community health center (HRSA). Call to confirm STD testing.

Agape Women's Health

1680 Dunn Ave, Jacksonville, FL 32218 · (904) 760-4904
Agape Community Health Center, Inc. · Community health center (HRSA). Call to confirm STD testing.

Duval Family Health Center - Enterprise

611 E Adams St, Jacksonville, FL 32202 · (904) 394-8100
I.M. Sulzbacher Center For The Homeless, INC · Community health center (HRSA). Call to confirm STD testing.

Duval Family Health Center - Village

5455 Springfield Blvd, Jacksonville, FL 32208 · (904) 394-4958
I.M. Sulzbacher Center For The Homeless, INC · Community health center (HRSA). Call to confirm STD testing.

Frequently asked questions

Where can I get STD testing in Jacksonville, FL?

This page lists 15 STD/HIV testing locations in Jacksonville, FL from the CDC NPIN directory, plus 7 community health centers that may offer testing. Call ahead to confirm services and hours.

Is there free STD testing in Jacksonville, FL?

7 of the 15 listed locations say they offer free STI and/or HIV testing. Eligibility can depend on age, income or residency. Many health departments and community health centers also use sliding-scale fees.

Do I need an appointment?

3 locations here say no appointment is required. Others may need an appointment. Call before you go.

Can I get a rapid HIV test?

10 locations here list rapid HIV testing, which usually gives results during the same visit.

Which tests should I ask for?

Common tests include chlamydia, gonorrhea, syphilis, HIV and sometimes herpes or hepatitis. Which ones you need depends on your situation, so a clinician can help you decide. CDC recommends everyone ages 13 to 64 get tested for HIV at least once.

Is STD testing confidential?

Health care providers must keep your health information private. Some public health clinics offer anonymous HIV testing. Ask the clinic about their privacy policy.

Data: CDC NPIN and HRSA. Page built 2026-11-03.