STD Testing in Orlando, Florida
45 STD and HIV testing locations in Orlando, including 25 that list free testing.
Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.
26Health
801 N Magnolia Ave, Orlando, FL 32803 · (321) 800-2922
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis C
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm
AIDS Healthcare Foundation – AHF Healthcare Center - Orlando
707 E Colonial Dr, Orlando, FL 32803 · (407) 204-7000
Free testing listedHIVRapid HIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Aspire Health Partners – CENTAUR Orange Counseling Center
106 W Columbia St, Orlando, FL 32806 · (407) 875-3700
Free testing listedHIVRapid HIVHepatitis CHepatitis B
Hours not listed
Bliss Cares
3901 E Colonial Dr, Orlando, FL 32803 · (407) 203-5984
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIV
Mon 7:00am – 7:00pm; Tue 7:00am – 7:00pm; Wed 7:00am – 7:00pm; Thu 7:00am – 7:00pm; Fri 7:00am – 7:00pm
CAN Community Health – at Miracle of Love
1301 W Colonial Dr, Orlando, FL 32804 · (407) 246-1946
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis C
Mon 8:30am – 5:30pm; Tue 8:30am – 5:30pm; Wed 8:30am – 5:30pm; Thu 8:30am – 5:30pm; Fri 8:30am – 12:30pm
Comprehensive Health Center – Orlando Center
1011 W Oak Ridge Rd, Orlando, FL 32809 · (407) 859-8797
Free testing listedChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:30am – 5:30pm; Tue 8:30am – 5:30pm; Wed 8:30am – 5:30pm; Thu 8:30am – 5:30pm; Fri 8:30am – 5:30pm
Crew Health
8601 Commodity Circle, Orlando, FL 32819 · (407) 605-2252
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 5:00pm; Tue 9:30am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm; Sat 9:00am – 5:00pm
Embrace Health – Embrace Health, Inc.
3100 Conway Road, Orlando, FL 32812 · (407) 259-4307
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 6:30pm; Tue 9:00am – 6:30pm; Wed 9:00am – 6:30pm; Thu 9:00am – 6:30pm; Fri 9:00am – 5:00pm
Harmony Healthcare – Orlando Office
189 S Orange Ave, Ste 1830, Orlando, FL 32801 · (407) 777-2022
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 4:00pm; Tue 9:00am – 4:00pm; Wed 9:00am – 4:00pm; Thu 9:00am – 4:00pm; Fri 9:00am – 12:00pm
HealthyMD – Orlando Clinic
130 E Marks St, 1st Fl, Orlando, FL 32803 · (407) 505-6435
Free testing listedChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:30am – 4:00pm; Tue 8:30am – 4:00pm; Wed 8:30am – 4:00pm; Thu 8:30am – 4:00pm; Fri 8:30am – 4:00pm
Inspire Healthcare Center
855 Outer Road, Ste 2B, Orlando, FL 32814 · (407) 299-3166
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHIVHepatitis CHepatitis B
Hours not listed
Invested Health Center
3162 Conway Rd, Orlando, FL 32812 · (407) 392-2273
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 6:00pm; Tue 9:00am – 6:00pm; Wed 9:00am – 6:00pm; Thu 9:00am – 6:00pm; Fri 9:00am – 6:00pm
Love Begins at Home
750 S Orange Blossom Trail, Ste 156, Orlando, FL 32805 · (407) 490-3809
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Hours not listed
Midway Specialty Care Center – Orlando Clinic
6000 Turkey Lake Rd, Ste 110, Orlando, FL 32819 · (407) 745-1171
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 4:30pm; Tue 9:00am – 4:30pm; Wed 11:00am – 6:30pm; Thu 9:00am – 4:30pm; Fri 9:00am – 2:30pm
Miracle of Love Incorporated – Stafford House
711 Seminole Ave, Orlando, FL 32804 · (407) 532-0070
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIV
Mon 12:30pm – 7:30pm; Tue 12:30pm – 7:30pm; Wed 12:30pm – 7:30pm; Thu 12:30pm – 7:30pm; Fri 2:30pm – 5:30pm
Northwest Family Health Center
5844 N Orange Blossom Trl, Orlando, FL 32810 · (407) 602-1100
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:30am – 5:30pm; Tue 8:30am – 5:30pm; Wed 8:30am – 5:30pm; Thu 8:30am – 5:30pm; Fri 8:30am – 5:30pm
OIC Inspired
1707 N Mills Ave, Orlando, FL 32803 · (407) 647-3960
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 7:00am – 5:00pm; Tue 7:00am – 5:00pm; Wed 7:00am – 5:00pm; Thu 7:00am – 5:00pm; Fri 7:00am – 4:00pm
Orange Blossom Family Health – Downtown Orlando
232 N Orange Blossom Trail, Orlando, FL 32805 · (407) 428-5751
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Orange Blossom Family Health – Ivey Lane Clinic
4426 Old Winter Garden Rd, Orlando, FL 32811 · (407) 428-5751
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHIVRapid HIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Orange Blossom Family Health – Evans Wellness Center
3100 Evans Trojans Way, Orlando, FL 32808 · (407) 428-5751
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHIVRapid HIV
Tue 8:00am – 5:00pm; Thu 8:00am – 5:00pm
Pineapple Healthcare
1724 33rd St, Ste 100, Orlando, FL 32839 · (407) 553-6336
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 2:00pm; Sat 10:00am – 2:00pm
Positive Assistance Incorporated – Inclusive Care Clinic
7156 Colonial Drive, Orlando, FL 32818 · (407) 412-6569
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Sun 1:00pm – 5:00pm; Mon 7:00am – 7:00pm; Tue 7:00am – 7:00pm; Wed 7:00am – 7:00pm; Thu 7:00am – 7:00pm; Fri 7:00am – 7:00pm; Sat 1:00pm – 5:00pm
Prevention X
17 N Summerlin Ave, Ste 100, Orlando, FL 32801 · (833) 773-7407
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm
The Center
942 N Mills Ave, Orlando, FL 32803 · (407) 228-8272
Free testing listedHIVRapid HIVHepatitis C
Sun 10:00am – 1:45pm; Mon 9:00am – 7:45pm; Tue 9:00am – 7:45pm; Wed 9:00am – 7:45pm; Thu 9:00am – 7:45pm; Fri 9:00am – 7:45pm; Sat 10:00am – 1:45pm
True Health – SouthSide Family Health Center
6101 Lake Ellenor Dr, Ste 105, Orlando, FL 32809 · (407) 322-8645
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 6:00pm; Wed 8:00am – 6:00pm; Thu 8:00am – 6:00pm; Fri 8:00am – 12:00pm
Advent Health Medical Group – Family Medicine at East Orlando
7975 Lake Underhill Rd, Ste 200, Orlando, FL 32822 · (407) 303-6830
ChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Community Health Centers Incorporated – Bithlo Family Medical
19108 E Colonial Dr, Orlando, FL 32820 · (407) 905-8827
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 7:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 1:00pm
Community Health Centers Incorporated – West Lakes Medical
710 S Tampa Ave, Ste 203, Orlando, FL 32805 · (407) 905-8827
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 7:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 1:00pm
Community Health Centers Incorporated – Pine Hills Family Health Center
840 Mercy Dr, Orlando, FL 32808 · (407) 905-8827
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis C
Mon 8:00am – 6:00pm; Tue 8:00am – 7:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 1:00pm
Community Health Centers Incorporated – Forest City Health Center
7900 Forest City, Orlando, FL 32810 · (407) 905-8827
Sliding scaleChlamydiaGonorrheaSyphilisHIVHepatitis C
Mon 8:00am – 6:00pm; Tue 8:00am – 7:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 1:00pm
Community Health Centers Incorporated – Meadow Woods Health Center
849 Greenway Professional Ct, Orlando, FL 32824 · (407) 905-8827
Sliding scaleChlamydiaGonorrheaSyphilisHIVHepatitis C
Mon 8:00am – 6:00pm; Tue 8:00am – 7:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 1:00pm
Florida Department of Health in Orange County – Central Health Center BLDG 1
832 W Central Blvd, Orlando, FL 32805 · (407) 858-1400
Sliding scaleChlamydiaGonorrheaHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Florida Department of Health in Orange County – Lila Mitchell Health Center
5151 Raleigh St, Ste B, Orlando, FL 32811 · (407) 723-5004
Sliding scaleChlamydiaGonorrheaSyphilisHIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Lake Conway Primary Care
5050 S Conway Rd, Ste 2, Orlando, FL 32812 · (407) 851-2790
ChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm
Legacy Family Medicine
11602 Lake Underhill Rd, Ste 119, Orlando, FL 32825 · (407) 781-1000
ChlamydiaGonorrheaSyphilisHIVRapid HIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm
Orlando Health – Medical Group Infectious Disease
1012 Lucerne Terr, Orlando, FL 32806 · (407) 423-1039
ChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 4:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:00pm
Pinero Preventive Medical Care
2766 E Colonial Dr, Orlando, FL 32803 · (407) 426-9693
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 7:00am – 6:00pm; Tue 7:00am – 6:00pm; Wed 7:00am – 6:00pm; Thu 7:00am – 6:00pm; Fri 7:00am – 6:00pm; Sat 9:00am – 2:00pm
Planned Parenthood of Florida – East Orlando Health Center
11500 University Blvd, Ste B, Orlando, FL 32817 · (407) 246-1788
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIV
Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm; Sat 8:00am – 4:00pm
Radiance Womens Center
1118 S Orange Ave, Ste 202, Orlando, FL 32806 · (407) 743-2100
ChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Hours not listed
Spektrum Health Incorporated
5205 S Orange Ave, Ste 110, Orlando, FL 32809 · (407) 454-1363
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:30am – 5:00pm; Tue 8:30am – 5:00pm; Wed 8:30am – 5:00pm; Thu 8:30am – 5:00pm; Fri 8:30am – 5:00pm
True Health – Lake Underhill Office
5730 Lake Underhill Rd, Orlando, FL 32807 · (407) 322-8645
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 6:00pm; Wed 8:00am – 6:00pm; Thu 8:00am – 6:00pm; Fri 8:00am – 12:00pm; Sat 8:00am – 12:00pm
True Health – Alafaya Office
11881-A E Colonial Dr, Orlando, FL 32826 · (407) 322-8645
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 6:00pm; Wed 8:00am – 6:00pm; Thu 8:00am – 6:00pm; Fri 8:00am – 12:00pm
True Health – Hoffner Office
5449 S Semoran Blvd, Ste 14, Orlando, FL 32822 · (407) 322-8645
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 6:00pm; Wed 8:00am – 6:00pm; Thu 8:00am – 6:00pm; Fri 8:00am – 12:00pm
UrgentMed Care
716 S Goldenrod Rd, Orlando, FL 32822 · (407) 459-4875
ChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 9:00am – 6:00pm; Tue 9:00am – 6:00pm; Wed 9:00am – 6:00pm; Thu 9:00am – 6:00pm; Fri 9:00am – 6:00pm; Sat 9:00am – 6:00pm; Sun 9:00am – 6:00pm
WellMed – Southwest Orlando Family Medicine PL
7400 Docs Grove Circle, Orlando, FL 32819 · (407) 352-9717
ChlamydiaGonorrheaSyphilisHIV
Mon 8:00am – 6:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm; Sat 8:00am – 12:00pm
Community health centers in Orlando
These federally funded health centers (HRSA) offer primary care, usually on a sliding fee scale. Their STD testing services are not confirmed in our data. Call to confirm STD testing.
Alafaya Health Center
11881 E Colonial Dr STE A, Orlando, FL 32826 · (407) 322-8645
Central Florida Family Health Center, Inc. · Community health center (HRSA). Call to confirm STD testing.
Frequently asked questions
Where can I get STD testing in Orlando, FL?
This page lists 45 STD/HIV testing locations in Orlando, FL from the CDC NPIN directory, plus 1 community health centers that may offer testing. Call ahead to confirm services and hours.
Is there free STD testing in Orlando, FL?
25 of the 45 listed locations say they offer free STI and/or HIV testing. Eligibility can depend on age, income or residency. Many health departments and community health centers also use sliding-scale fees.
Do I need an appointment?
12 locations here say no appointment is required. Others may need an appointment. Call before you go.
Can I get a rapid HIV test?
33 locations here list rapid HIV testing, which usually gives results during the same visit.
Which tests should I ask for?
Common tests include chlamydia, gonorrhea, syphilis, HIV and sometimes herpes or hepatitis. Which ones you need depends on your situation, so a clinician can help you decide. CDC recommends everyone ages 13 to 64 get tested for HIV at least once.
Is STD testing confidential?
Health care providers must keep your health information private. Some public health clinics offer anonymous HIV testing. Ask the clinic about their privacy policy.
Data: CDC NPIN and HRSA. Page built 2026-11-03.