STD Testing in Louisville, Kentucky
21 STD and HIV testing locations in Louisville, including 6 that list free testing.
Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.
Louisville Metro Department of Public Health and Wellness – Specialty Clinic
914 E Broadway, Ste 102, Louisville, KY 40204 · (502) 574-6699
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHIVHepatitis C
Mon 8:00am – 4:00pm; Tue 8:00am – 4:00pm; Wed 8:00am – 4:00pm; Thu 8:00am – 4:00pm; Fri 8:00am – 4:00pm
Louisville Pride Foundation
1244 S 3rd St, Louisville, KY 40203 · (502) 498-4298
Free testing listedHIVRapid HIV
Tue 11:59am – 7:00pm; Wed 11:59am – 7:00pm; Thu 11:59am – 7:00pm; Fri 11:59am – 7:00pm; Sat 11:59am – 6:00pm
Music City PrEP Clinic – Louisville Clinic
1101 E Washington St, Ste A, Louisville, KY 40206 · (502) 546-6069
Free testing listedSliding scaleChlamydiaGonorrheaSyphilisHIVHepatitis CHepatitis B
Hours not listed
Redeemer Lutheran Church
3640 River Park Dr, Louisville, KY 40211 · (502) 776-5945
Free testing listedHIVRapid HIV
Hours not listed
Solutions Health
1620 Arthur St, Ste 119, Louisville, KY 40203 · (502) 208-8541
Free testing listedChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Wed 10:00am – 5:00pm; Thu 10:00am – 5:00pm; Fri 10:00am – 5:00pm
Solutions Health
1801 Portland Ave, Louisville, KY 40203 · (502) 208-8541
Free testing listedChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Tue 10:00am – 2:00pm
Family Health Centers Incorporated – East Broadway Health Center
834 E Broadway, Louisville, KY 40204 · (502) 583-1981
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 9:00pm; Wed 8:00am – 9:00pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm; Sat 8:00am – 12:00pm
Family Health Centers Incorporated – Portland Avenue Clinic
2215 Portland Ave, Louisville, KY 40212 · (502) 774-8631
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 4:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 9:00pm; Fri 8:00am – 4:30pm; Sat 8:00am – 12:00pm
Family Health Centers Incorporated – Americana Health Center
4805 Southside Dr, Louisville, KY 40214 · (502) 772-8860
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 4:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm
Family Health Centers Incorporated – Phoenix Health Care for the Homeless
712 E Muhammad Ali Blvd, Louisville, KY 40202 · (502) 568-6972
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 7:30am – 4:00pm; Tue 7:30am – 4:00pm; Wed 7:30am – 4:00pm; Thu 7:30am – 4:00pm; Fri 7:30am – 4:00pm
Family Health Centers Incorporated – Iroquois Center
4100 Taylor Blvd, Louisville, KY 40215 · (502) 366-4747
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 8:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm
Norton Healthcare Infectious Diseases Institute
4950 Norton Healthcare Blvd, Ste 303, Louisville, KY 40241 · (502) 446-6434
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Hours not listed
Norton Healthcare Infectious Diseases Institute – Downtown Clinic
601 Floyd St, Ste 604, Louisville, KY 40202 · (502) 446-6434
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Hours not listed
NuLease Medical Solutions
5722 Outer Loop, Louisville, KY 40219 · (502) 492-7455
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 6:00pm; Tue 8:00am – 6:00pm; Wed 8:00am – 6:00pm; Thu 8:00am – 6:00pm
Park DuValle Community Health Center Incorporated – Main Office
3015 Wilson Ave, Louisville, KY 40211 · (502) 774-4401
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis C
Mon 8:00am – 5:00pm; Tue 8:00am – 8:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm; Sat 9:00am – 12:00pm
Park DuValle Community Health Center Incorporated – Russell Neighborhood Health Center
1425 W Broadway, Louisville, KY 40203 · (502) 584-2992
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Park DuValle Community Health Center Incorporated – Park DuValle at Newburg
3828 Bardstown Rd, Louisville, KY 40218 · (502) 479-8930
Sliding scaleChlamydiaGonorrheaSyphilisHIV
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
Planned Parenthood Great Northwest Hawaii Alaska Indiana Kentucky – Louisville Health Center
842 S 7th St, Louisville, KY 40203 · (800) 769-0045
Sliding scaleChlamydiaGonorrheaHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 4:00pm; Tue 10:00am – 6:00pm; Wed 8:00am – 4:00pm; Thu 8:00am – 4:00pm; Fri 8:00am – 4:00pm
Shawnee Christian Healthcare Center Incorporated
234 Amy Ave, Louisville, KY 40212 · (502) 778-0001
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:30am – 5:00pm; Tue 8:30am – 5:00pm; Wed 8:30am – 5:00pm; Thu 8:30am – 7:00pm; Fri 8:30am – 5:00pm
University of Louisville Hospital – Hep C Center
401 E Chestnut St, Ste 550, Louisville, KY 40202 · (502) 588-0425
ChlamydiaGonorrheaSyphilisHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm
University of Louisville Physicians – Ambulatory Care Bldg
550 S Jackson St, 2nd Fl, Louisville, KY 40202 · (502) 561-8844
Sliding scaleHIV
Mon 8:00am – 4:00pm; Tue 8:00am – 4:00pm; Wed 8:00am – 4:00pm; Thu 8:00am – 4:00pm; Fri 8:00am – 4:00pm
Community health centers in Louisville
These federally funded health centers (HRSA) offer primary care, usually on a sliding fee scale. Their STD testing services are not confirmed in our data. Call to confirm STD testing.
FHC - Sheehan Landing
3501 Sheehan Landing Dr, Louisville, KY 40219 · (502) 358-2310
Family Health Centers, Inc. · Community health center (HRSA). Call to confirm STD testing.
Family Health Centers - West Market
2500 W Market St, Louisville, KY 40212 · (502) 778-8400
Family Health Centers, Inc. · Community health center (HRSA). Call to confirm STD testing.
Family Health Centers, Inc. - Wayside Hotel Louisville
120 W Broadway, Louisville, KY 40202 · (502) 772-8662
Family Health Centers, Inc. · Community health center (HRSA). Call to confirm STD testing.
Frequently asked questions
Where can I get STD testing in Louisville, KY?
This page lists 21 STD/HIV testing locations in Louisville, KY from the CDC NPIN directory, plus 3 community health centers that may offer testing. Call ahead to confirm services and hours.
Is there free STD testing in Louisville, KY?
6 of the 21 listed locations say they offer free STI and/or HIV testing. Eligibility can depend on age, income or residency. Many health departments and community health centers also use sliding-scale fees.
Do I need an appointment?
4 locations here say no appointment is required. Others may need an appointment. Call before you go.
Can I get a rapid HIV test?
8 locations here list rapid HIV testing, which usually gives results during the same visit.
Which tests should I ask for?
Common tests include chlamydia, gonorrhea, syphilis, HIV and sometimes herpes or hepatitis. Which ones you need depends on your situation, so a clinician can help you decide. CDC recommends everyone ages 13 to 64 get tested for HIV at least once.
Is STD testing confidential?
Health care providers must keep your health information private. Some public health clinics offer anonymous HIV testing. Ask the clinic about their privacy policy.
Data: CDC NPIN and HRSA. Page built 2026-11-03.