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STD Testing in Owensboro, Kentucky

5 STD and HIV testing locations in Owensboro, including 1 that list free testing.

Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.

Matthew 25 AIDS Services Incorporated – Owensboro

1901 Leitchfield Rd, Ste A, Owensboro, KY 42303 · (270) 826-0200
Free testing listedSliding scaleHIVRapid HIVHepatitis C
Tue 8:00am – 4:30pm; Thu 8:00am – 3:30pm

Kentucky Cabinet for Health and Family Services – Green River District Health Department

1600 Breckenridge St, Owensboro, KY 42303 · (270) 686-7744
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis C
Mon 8:00am – 4:30pm; Tue 8:00am – 4:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm

Owensboro Health – Owensboro Health Family Medicine in Owensboro

811 E Parish Ave, Owensboro, KY 42303 · (270) 684-1145
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

Owensboro Health – Owensboro Health Family Medicine in Owensboro

1325 Triplett St, Triplett Medical Bldg, Owensboro, KY 42303 · (270) 686-8500
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 7:00am – 5:00pm; Tue 7:00am – 5:00pm; Wed 7:00am – 5:00pm; Thu 7:00am – 5:00pm; Fri 7:00am – 5:00pm

Owensboro Health – Owensboro Health Medical Group Infectious Disease

1301 Pleasant Valley Rd, Ste 401, Owensboro, KY 42303 · (270) 417-7850
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 12:00pm

Community health centers in Owensboro

These federally funded health centers (HRSA) offer primary care, usually on a sliding fee scale. Their STD testing services are not confirmed in our data. Call to confirm STD testing.

Audubon Area Community Care Clinic

1620 Frederica St, Owensboro, KY 42301 · (270) 686-6040
Audubon AREA Community Care Clinic, INC · Community health center (HRSA). Call to confirm STD testing.

Audubon Area Community Care Clinic - Benttree

4215 Benttree Dr, Owensboro, KY 42301 · (270) 686-6040
Audubon AREA Community Care Clinic, INC · Community health center (HRSA). Call to confirm STD testing.

Audubon Area Community Care Clinic - Daniel Pitino Shelter

501 Walnut St, Owensboro, KY 42301 · (270) 686-6040
Audubon AREA Community Care Clinic, INC · Community health center (HRSA). Call to confirm STD testing.

Audubon Area Community Care Clinic - Owensboro - Business Office

1721 W 5th St, Owensboro, KY 42301 · (270) 686-6040
Audubon AREA Community Care Clinic, INC · Community health center (HRSA). Call to confirm STD testing.

Regional Health Care Affiliates, Inc. dba. Health First Community Health Center- Neel Clinic

2816 Veach Rd, Ste 205, Owensboro, KY 42303 · (270) 926-9821
Regional Health Care Affiliates, Inc. · Community health center (HRSA). Call to confirm STD testing.

Frequently asked questions

Where can I get STD testing in Owensboro, KY?

This page lists 5 STD/HIV testing locations in Owensboro, KY from the CDC NPIN directory, plus 5 community health centers that may offer testing. Call ahead to confirm services and hours.

Is there free STD testing in Owensboro, KY?

1 of the 5 listed locations say they offer free STI and/or HIV testing. Eligibility can depend on age, income or residency. Many health departments and community health centers also use sliding-scale fees.

Do I need an appointment?

Appointment rules vary by location. Call before you go.

Can I get a rapid HIV test?

4 locations here list rapid HIV testing, which usually gives results during the same visit.

Which tests should I ask for?

Common tests include chlamydia, gonorrhea, syphilis, HIV and sometimes herpes or hepatitis. Which ones you need depends on your situation, so a clinician can help you decide. CDC recommends everyone ages 13 to 64 get tested for HIV at least once.

Is STD testing confidential?

Health care providers must keep your health information private. Some public health clinics offer anonymous HIV testing. Ask the clinic about their privacy policy.

Data: CDC NPIN and HRSA. Page built 2026-11-03.