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STD Testing in Knoxville, Tennessee

16 STD and HIV testing locations in Knoxville, including 3 that list free testing.

Verify with the clinic before visiting. Hours, services, fees and appointment rules change. Call ahead to confirm. This directory is for information only and is not medical advice. In an emergency, call 911.

Choice Health Network a Positively Living Company – Knoxville

1925 Ailor Ave, Knoxville, TN 37921 · (865) 525-1540
Free testing listedChlamydiaGonorrheaSyphilisHIVRapid HIVHepatitis CHepatitis B
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 5:00pm

Hope Resource Center

2700 Painter Ave, Knoxville, TN 37919 · (865) 525-4673
Free testing listedChlamydiaGonorrheaSyphilisHerpesHIV
Mon 9:00am – 5:00pm; Tue 9:00am – 5:00pm; Wed 9:00am – 5:00pm; Thu 9:00am – 5:00pm; Fri 9:00am – 1:00pm

Samaritan Ministry

6300 Deane Hill Dr, Knoxville, TN 37919 · (865) 450-1000
Free testing listed
Hours not listed

AFC Urgent Care

6108 Kingston Pike, Knoxville, TN 37919 · (865) 539-9299
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Sun 8:00am – 5:00pm; Mon 8:00am – 8:00am; Tue 8:00am – 8:00am; Wed 8:00am – 8:00am; Thu 8:00am – 8:00am; Fri 8:00am – 8:00am; Sat 8:00am – 5:00pm

AFC Urgent Care

5150 N Broadway, Knoxville, TN 37918 · (865) 549-5111
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 8:00pm; Tue 8:00am – 8:00pm; Wed 8:00am – 8:00pm; Thu 8:00am – 8:00pm; Fri 8:00am – 8:00pm; Sat 8:00am – 5:00pm; Sun 8:00am – 5:00pm

AFC Urgent Care

7564 Chapman Hwy, Knoxville, TN 37920 · (865) 951-1103
ChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 8:00pm; Tue 8:00am – 8:00pm; Wed 8:00am – 8:00pm; Thu 8:00am – 8:00pm; Fri 8:00am – 8:00pm; Sat 8:00am – 5:00pm; Sun 8:00am – 5:00pm

Knox County Health Department – West Clinic

9000 Executive Park Dr, Ste A301B, Knoxville, TN 37923 · (865) 215-5950
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 6:00pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm

Knox County Health Department

140 Dameron Ave, Knoxville, TN 37917 · (865) 215-5000
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 4:30pm; Tue 8:00am – 4:30pm; Wed 8:00am – 4:30pm; Thu 8:00am – 4:30pm; Fri 8:00am – 4:30pm

Maltman Medical Center

9051 Executive Park Dr, Ste 500, Knoxville, TN 37923 · (865) 337-7793
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 1:00pm

Oak Street Health – East Knoxville

4221 Asheville Hwy, Knoxville, TN 37914 · (865) 936-9051
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

Planned Parenthood of Tennessee and North Mississippi – Knoxville Health Center

710 N Cherry St, Knoxville, TN 37914 · (866) 711-1717
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIV
Mon 9:00am – 6:00pm; Tue 9:00am – 4:00pm; Wed 9:00am – 4:00pm; Thu 9:00am – 6:00pm; Fri 9:00am – 4:00pm

River Valley Health – Knoxville County Center City Health Center

2018 Western Ave, Knoxville, TN 37921 · (865) 544-0406
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

River Valley Health – Knox County 5th Ave Health Center

538 W 5th Ave, Knoxville, TN 37917 · (865) 525-2104
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVRapid HIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

River Valley Health – Knox County East Knox Health Center

2202 Martin Luther King Jr Ave, Knoxville, TN 37915 · (865) 522-6097
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

River Valley Health – Knox County Lonsdale Health Center

1754 Texas Ave, Knoxville, TN 37921 · (865) 934-6165
Sliding scaleChlamydiaGonorrheaSyphilisHerpesHIVHepatitis CHepatitis B
Mon 8:00am – 5:00pm; Tue 8:00am – 5:00pm; Wed 8:00am – 5:00pm; Thu 8:00am – 5:00pm; Fri 8:00am – 5:00pm

Summit Medical Group – Farragut Family Practice

11130 Kingston Pike, Stes 7 and 8, Knoxville, TN 37934 · (865) 675-1953
ChlamydiaGonorrheaSyphilisHIVHepatitis CHepatitis B
Mon 7:30am – 6:00pm; Tue 7:30am – 6:00pm; Wed 7:30am – 6:00pm; Thu 7:30am – 6:00pm; Fri 7:30am – 6:00pm

Community health centers in Knoxville

These federally funded health centers (HRSA) offer primary care, usually on a sliding fee scale. Their STD testing services are not confirmed in our data. Call to confirm STD testing.

CHS Cherokee Mills

2240 Sutherland Ave, Knoxville, TN 37919 · (865) 544-0406
Cherokee Health Systems · Community health center (HRSA). Call to confirm STD testing.

CHS First Creek at Austin

1250 Elston Turner Dr, Knoxville, TN 37915 · (865) 544-0406
Cherokee Health Systems · Community health center (HRSA). Call to confirm STD testing.

CHS Mechanicsville

601 College St, Knoxville, TN 37921 · (865) 934-6120
Cherokee Health Systems · Community health center (HRSA). Call to confirm STD testing.

CHS Oakwood

1515 Saint Mary St, Knoxville, TN 37917 · (865) 934-6125
Cherokee Health Systems · Community health center (HRSA). Call to confirm STD testing.

Frequently asked questions

Where can I get STD testing in Knoxville, TN?

This page lists 16 STD/HIV testing locations in Knoxville, TN from the CDC NPIN directory, plus 4 community health centers that may offer testing. Call ahead to confirm services and hours.

Is there free STD testing in Knoxville, TN?

3 of the 16 listed locations say they offer free STI and/or HIV testing. Eligibility can depend on age, income or residency. Many health departments and community health centers also use sliding-scale fees.

Do I need an appointment?

5 locations here say no appointment is required. Others may need an appointment. Call before you go.

Can I get a rapid HIV test?

8 locations here list rapid HIV testing, which usually gives results during the same visit.

Which tests should I ask for?

Common tests include chlamydia, gonorrhea, syphilis, HIV and sometimes herpes or hepatitis. Which ones you need depends on your situation, so a clinician can help you decide. CDC recommends everyone ages 13 to 64 get tested for HIV at least once.

Is STD testing confidential?

Health care providers must keep your health information private. Some public health clinics offer anonymous HIV testing. Ask the clinic about their privacy policy.

Data: CDC NPIN and HRSA. Page built 2026-11-03.